CCK8 proliferation assays, flow cytometry-based apoptosis analysis, and cell cycle analysis revealed that compared with control cells, SCC9 cells with NMT1 knockdown (shNMT1) presented no significant differences in proliferative capacity, apoptosis rate, or cell cycle distribution (Fig S1H-J), suggesting that NMT1 deficiency does not directly affect the autonomous growth characteristics of tumor cells. Here, NMT1 is linked to neoplasm.