Consistent with these findings, the expression profile driven by MTHFD2 downregulation in bladder cancer cells showed an enrichment of the phosphatidylinositol 3-kinase (PI3K)/AKT pathway and decreased the protein levels of phospho-PI3K/PI3K and phospho-AKT/AKT, demonstrating that MTHFD2 depletion suppresses the PI3K/AKT pathway72. The gene discussed is AKT1; the disease is urinary bladder cancer.