Three proteins, caldesmon (CALD), heterogeneous nuclear ribonucleoprotein M (HNRNPM) and tropomyosin 1 (TPM1) were unique discriminative between LSCC and RSCC tumour cell-rich HND regions, however could not be identified as discriminative when the whole LSCC and RSCC tumour region were analysed. Here, CALD1 is linked to tooth and nail syndrome.