When iDISCO tissue clearing and 3D lightsheet imaging were applied to a preparation of the lumbar SPC in which meninges were preserved (see Materials and Methods), we found that Lyve1 density, although highly variable, trended higher in ALS tissue than in control tissue (Fig. 7F,H) (NC, 0.686±0.133%; ALS, 1.011±0.245%; P=0.278). Here, LYVE1 is linked to amyotrophic lateral sclerosis.