MEF2C and neoplasm: Investigating primary embryonal and alveolar RMS patient tumor tissues using single cell RNA sequencing revealed that tumors exhibited subpopulations of paraxial mesoderm expressing MEOX2 and PAX3, myoblasts expressing MYF5 and MSC, and myocytes expressing MYOG and MEF2C. In addition, there were differences between alveolar RMS and embryonal RMS, where a greater percentage of tumor cells in alveolar RMS exhibited myocyte-like state expressing MYOG, and a lesser percentage exhibited paraxial mesoderm with MEOX2 expression (132).