Knocking down PAX3::FOXO1 in human RMS cells resulted in decreased cell proliferation, lower motility, increased protein levels of Desmin and Myosin Heavy Chain (MHC), down-regulation of mRNA levels of MYOD1, MYF5, MRF4, and increased myogenin protein levels, highlighting the importance of PAX3::FOXO1 in tumor maintenance and in suppressing myogenic differentiation (49). This evidence concerns the gene FOXO1 and neoplasm.