ATP6V0B and familial pancreatic carcinoma: To validate ATP6V0B as a pancreatic tumor marker from circulating EVs for liquid biopsy, we collected plasma samples (N = 22) from 16 early-stage pancreatic cancer individuals, of which 6 had matched pancreas tumor tissues, and 6 healthy samples (Additional file 1: Table S6 for patient information) for quantitative polymerase chain reaction (qPCR) detection.