We further applied a population comparison of ATP6V0B gene expression using pancreatic tissue from UCSC’s Xena database to compare non-diseased Genome-Tissue Expression (GTEX) [76] and TCGA’s PDAC (Additional file 1: Fig. S23), which confirmatively showed a significant increase of specific ATP6V0B exon expression in the cancer group (Fig. 6c), consistent with the EVs isolated by ExCy (Fig. 6d, and Additional file 1: Fig. S6 and S23). This evidence concerns the gene ATP6V0B and cancer.