In DMP dataset, “CD28 + CD45RA + CD8 + T cell %”, “CD28 + CD45RA + CD8dim T cell %”, “CD45RA + CD8 + T cell %”, “CD86 on myeloid Dendritic Cell” exhibited mediation in the causal association between Alistipes indistinctus and CKD, with a mediation proportion of 1.59, 3.05, 1.50, and 11.72(p < 0.05). The gene discussed is CD28; the disease is chronic kidney disease.