Our mediator analysis also suggests that CD86 on myeloid Dendritic Cell is the positive mediator effect of Alistipes indistinctus to CKD and “CD28 + CD45RA + CD8 + T cell %”, “CD28 + CD45RA + CD8dim T cell %”, “CD45RA + CD8 + T cell %” is the negative effect between Alistipes indistinctus, Alistipes putredinis to CKD. This evidence concerns the gene CD28 and chronic kidney disease.