UHRF1 and cancer: This inhibition of DNMT1 led to an increase of SA-β-gal positivity in all cell lines, along with a markedly decrease capacity to incorporate EdU during a 20-h pulse (Fig. 1M and Supplementary Fig. 1J) further validating the hypothesis that DNA demethylation induced by UHRF1 or DNMT1 removal triggers senescence, a phenomenon consistent in multiple cancer cell types as well as with various methods of depletion.