TIGIT and neoplasm: QPCR analysis of treated T cells indicated that the tumor cell supernatant (TC group) elevated the expression of LAG, Tigit, and PD-1 genes, with these increases being mitigated in the TC-BAM15 group (Fig. 2B), suggesting that tumor cell secretions contribute to T cell exhaustion, which BAM15 treatment alleviated by altering these secretions.