Genome-wide profiling of histone modifications, ASCL1 and NEUROD1 occupancy, gene and miRNA expression, and gene co-expression patterns determined using the CUT&Tag assay, RNA-seq, and small RNA-seq analyses revealed that ASCL1 and NEUROD1 regulate distinct target genes and miRNAs in SCLC-A/N cells. Here, NEUROD1 is linked to small cell lung carcinoma.