Subsequent investigations revealed substantial negative correlations between GNGT1 and tumour mutational burden(TMB), microsatellite instability(MSI), immune cell infiltration, immune cell gene marker expression and immunological checkpoint expression in patients with STAD.GNGT1 is a reliable biomarker in patients with GC that also has an immunomodulatory function in this disease and may accelerate GC development by suppressing the infiltration of T cells, dendritic cells, M1 macrophages and B cells. The gene discussed is GNGT1; the disease is neoplasm.