CD8A and neoplasm: In contrast, R-MHAs loaded with 10–60 ng/mL Rot did not cause lethality in M0-BMDMs or naive CD8+ T cells, R-MHAs carrying 10–50 ng/mL Rot did not affect the viability of activated CD8+ T cells, and higher concentrations only mildly weakened the cell viabilities, manifesting that tumor cells were more sensitive to the inhibition of Asn synthesis than other cell types (Fig. 3c and Supplementary Fig. 10).