HLA-A and melanoma: When we stimulated these cells from the day +43 PBMC sample from NYSCT-03 with the human melanoma cell line M257-A2, which is NY-ESO-1-positive and HLA-A:02:01-positive, we found significant elevations in secreted interferon-gamma, compared to the T cells cultured alone or with M257 cells, which are positive for NY-ESO-1 but negative for HLA-A:02:01 (Supplementary Fig. 7).