Furthermore, PKIB knockdown significantly inhibited EMT, migration, invasion, and proliferation of 5637 (basal subtype), T24 (mixed subtype), and RT112 (luminal subtype) cells (Fig. 2 and Fig. S1), suggesting that the oncogenic role of PKIB in different subtypes of BLCA cells is conserved. This evidence concerns the gene PKIB and bladder transitional cell carcinoma.