Furthermore, the transcription factor MYCN is implicated in regulating the overexpression of PKIB. These results illuminate the oncogenic role of PKIB and reveal an innovative signaling axis involving MYCN-PKIB-PKA-HSP27 in BLCA, enhancing our understanding of the intricate cellular pathways and regulatory mechanisms mediated by PKA kinases while providing valuable insights into PKIB as a potential novel therapeutic target for targeted intervention in bladder tumors. This evidence concerns the gene HSPB1 and bladder transitional cell carcinoma.