SLAMF1 and leukemia: Generally, purified HSCs (Lin− Sca-1+ c-kit+ CD150+= LSK-CD150 cells) or bone marrow cells from mice pre-treated with 5-fluorouracil (5-FU) to enrich bone marrow in hematopoietic stem/progenitor cells (HSPC) are utilized for retroviral transduction carrying leukemia specific oncogenes (Figure 4A).