To determine whether this interaction also takes place in cellulo, we conducted co-immunoprecipitation assays of the endogenous proteins in HCT-116 CRC cells, which express high levels of SMYD3 and c-MYC and exhibit marked stemness properties, such as sphere- and soft agar colony-forming abilities, as well as increased expression of several CRC-SC markers.25 Our findings confirmed that SMYD3 physically interacts with c-MYC in this CRC cell model (Fig. 1d). This evidence concerns the gene MYC and colorectal carcinoma.