Subsequently, to get further insights into the role and biological significance of c-MYC K158 and K163 methylation, we generated c-MYC_K158A and c-MYC_K163A knock-in (KI) HCT-116 CRC cell lines by using the CRISPR/Cas9 system for genome editing (Supplementary Fig. 5e). Here, MYC is linked to colorectal carcinoma.