Based on our data showing a direct interaction between SMYD3 and c-MYC, and considering that c-MYC exerts its oncogenic role by promoting cancer-related gene expression, we performed a ddPCR assay to investigate c-MYC stemness-related transcriptional activity upon loss of SMYD3 function, either genetically determined or pharmacologically induced. This evidence concerns the gene SMYD3 and cancer.