To explore the relevance of STYK1 phosphorylation and AP1/2-mediated STYK1 membrane trafficking in pancreatic cancer cell proliferation, we established isogenic PANC-1 cell lines ectopically expressing wild-type STYK1 or its phosphoregulatory variants (phosphomimetic Y191D, non-phosphorylatable Y191F, and SS1/2/3) by employing a G418-based selection strategy in a STYK1 depletion context. Here, STYK1 is linked to familial pancreatic carcinoma.