JAK2 and acquired polycythemia vera: At King Faisal Specialist Hospital and Research Centre in Riyadh, Alghasham et al used Sanger sequencing to identify mutations in JAK2 exons 12 to 15 among patients harboring mutations linked to PV, essential thrombocythemia and MF.[9] Initially, this study involved 1811 samples, but 1706 samples were analyzed for JAK2 mutations as 105 of the samples were deemed duplicates or insufficient.