Given the observed downregulation of Gnb5 in AD and its negative correlation with Braak stages (S1B Fig), along with evidence that Gnb5 deficiency in excitatory neurons causes memory deficits (Fig 2), we sought to evaluate whether overexpressing Gnb5 could mitigate cognitive deficits in 5xFAD mice. Here, GNB5 is linked to Alzheimer disease.