We also showed that the Us3 A326V mutation, which significantly impaired pathogenic manifestations in and around the eyes, viral replication in the TGs and brains, and viral pathogenicity in mice following ocular infection, although the mutation had no effect on viral replication in eyes and viral entry into the TGs, altered the phosphorylation levels of p167, p144, and p37. This evidence concerns the gene RPS3 and eye infection.