The aim of the study was to analyze gene expressions of NLRP3, PTGS2/COX-2, IL-6, IL-1β, IL-10, IL-17A, IL-18, IL-21, IL-23, TNFα, IFNγ, and macrophage inflammatory protein-2 (MIP-2) in the kidneys of mice with systemic acanthamoebiasis. The gene discussed is PTGS2; the disease is Acanthamoeba infectious disease.