Using the established cortical organoid protocol (Figure 1A), we generated cortical organoids from iPSCs, HGADFN167 derived from a patient with HGPS, and HGADFN168 derived from a healthy paternal control, both obtained from the Progeria Research Foundation Cell and Tissue Bank (Figure 1B), and examined progerin expression and irregular nuclear morphology to investigate whether these organoids exhibited hallmarks of HGPS pathology (Goldman et al. 2004). The gene discussed is LMNA; the disease is Hutchinson-Gilford progeria syndrome.