CD274 and neoplasm: Additionally, TILs in Neo‐CRCVAS‐treated mice exhibited high expression of PD‐1 but low expression of activation marker 4‐1BB (Figure 2E); correspondingly, the obvious upregulation of PD‐L1 and downregulation of MHCI were confirmed by flow cytometry analysis in tumor tissues of Neo‐CRCVAS‐treated mice (Figure 2F,G).