LDHA and cancer: ALDH activity at baseline was higher than reported in literature for OVCAR3 and OVCAR4, which have reported levels of ALDH activity of 3.2–26.5% of cells and 4.1% of cells, respectively, whereas OVCAR8 tumoroids remained with low levels of ALDH, more consistent with literature values of 0.49–1.9% of cells.[54,99–101] The higher ALDH activity in our tri-component tumoroid platform is likely due to the tumoroids enriching for cancer stem-like cells in the attachment-free suspended fluidic environment.