We used six TSC lines derived in our lab from 6–8 week gestation placental tissues (including the 3 subjected to scRNA-seq above), performing flow cytometry for all 5 of these markers, and found these cells to almost uniformly express EGFR (95.5±3%), ITGA6 (94.6±2.4%), ITGA5 (98.6±0.8%), and ITGA2 (99.6±0.4%), with a small fraction co-expressing HLA-G (9.4 ±6.2%) (Fig. 4g). This evidence concerns the gene ITGA6 and tuberous sclerosis.