LIMD1 and Alzheimer disease: For instance, in the comparison of aging-associated CpGs with those reported in prior AD studies, cg02336827, located in the promoter region of the LIMD1 gene and hypomethylated with age, emerged as one of the most significant CpGs, showing a P-value of 4.63×10−7 and consistent hypomethylation in AD (Supplementary Table 8).55LIMD1 encodes a scaffold protein involved in key cellular functions, including transcriptional repression, microRNA-mediated gene silencing, and cytoskeletal organization.