This study aims to establish a prognostic diagnostic model for acute myeloid leukemia based on Ara-C resistance-related genes and investigate the mechanism by which the key gene S100A4(S100 calcium binding protein A4), activated by the transcription factor NR6A1 (nuclear receptor subfamily 6 group A member 1), mediates Ara-C resistance in acute myeloid leukemia cells through the p53 cell cycle signaling pathway. Here, NR6A1 is linked to acute myeloid leukemia.