To assess whether ALS‐/FTD‐associated TDP‐43 mutations influence GAP43 splicing, we co‐transfected pEGFP‐4a1 with various mutant TDP‐43 constructs, including TDP‐43A90V, TDP‐43K263E, TDP‐43G295S, TDP‐43G295R, TDP‐43M311V, TDP‐43A315T, TDP‐43Q311K, and TDP‐43M337V, and analyzed RT‐PCR results. Here, TARDBP is linked to amyotrophic lateral sclerosis.