To determine whether ALS‐/FTD‐associated TDP‐43 mutations influence intracellular localization, we overexpressed TDP‐43A90V, TDP‐43K263E, TDP‐43G295S, TDP‐43G295R, TDP‐43M311V, TDP‐43A315T, TDP‐43Q331K, and TDP‐43M337V in HeLa cells, followed by immunofluorescence staining and nucleocytoplasmic fractionation (Figure S5D, Supporting Information). The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.