Supplementary Fig. S3 shows the evaluation of Monocyte DIRECT LS-TA using CTSS as the denominator gene. Similarly, Wilcoxon group-wise P values ranged from 1.3x10−6 (DIRECT LS-TA NFKBIZ) to 6.9x10−12 (DIRECT LS-TA PFKFB3). AUC of MoM of DIRECT LS-TA of these 6 additional target genes was over 0.8. The results suggested that both denominator genes (PSAP and CTSS) produced similar results and performance in terms of detection of host response towards bacterial infection. This evidence concerns the gene PSAP and bacterial infectious disease.