A commonly discussed mechanism for spontaneous regression is anti-tumor immune responses.3,4 ScRNA-seq of established tumors from Th-MYCN mice has demonstrated infiltration by macrophages, T cells, B cells, dendritic cells, and other myeloid cells.37 However, our data showed that, while NKT cells and macrophages were present in Th-MYCN+/− SMG, they appeared in low proportions and also in WT mice (Figure 2C and D). This evidence concerns the gene MYCN and neoplasm.