To evaluate the role of TcES glycans in M2 macrophage generation, we conducted flow cytometry to detect F4/80, PD-L2, PD-L1, and MR in the peritoneal cavity and lamina propria of colitis mice treated with iTcES, TcES depleted of carbohydrates, and TcES depleted of proteins. The gene discussed is PDCD1LG2; the disease is colitis.