To further explore the repercussions of glutamine metabolism on the dynamic interaction between tumor cells and CD8+ T cells, we constructed a 3D T‐cell‐incorporated CSCC organoid model (Figure7A), which was established by coculturing CSCC organoids with T cells isolated from PBMCs, embedding them within a Matrigel matrix to facilitate the formation of 3D T‐cell‐incorporated CSCC organoids. Here, CD8A is linked to neoplasm.