To validate the expression changes of the feature genes in HNSCC, 24 pairs of tumor and adjacent noncancerous tissues were collected, and qPCR was performed for verification. The results showed no significant expression changes for PRKAA and GRIP2 in head and neck tumors (Figures 9A, B), whereas CHGB exhibited a noticeable upregulation, and SLC7A5 showed downregulation in head and neck tumors (Figures 9C, D). This evidence concerns the gene CHGB and head and neck squamous cell carcinoma.