Flow cytometry assessed BsAb binding to A431 (CD16A-/GPC3-), GPC3+ hepatocellular carcinoma (HCC) lines (HepG2, Huh7, Hep3B), A431-GPC3 (G1), PBMCs, and A431-CD16A cells. Here, GPC3 is linked to hepatocellular carcinoma.