In vitroRAW264.7 and HER2 beadsAnd GL261-H glioblastoma cell lineIn vivoBSG mice model with tumors derived from GL261-H cells with RP-182 nanoparticles containing CARAn orthotopicBSG PDX (patient-derived xenograft) model established by intracranial injectionof patient-derived brainstem glioma cells into huHSCNOG-EXL mice. The gene discussed is ERBB2; the disease is brain stem glioma.