Assessments included serum SOD activity, cardiac tissue ROS levels, cardiomyocyte apoptosis rates (TUNEL assay), mRNA and protein expression of IL-1β, TNF-α, and IL-6 (qPCR, Western blot), histopathological evaluation of myocardial tissue morphology and inflammatory infiltration (H&E staining), myocardial infarction size (TTC staining), and cardiac function parameters (contractility, diastolic function). The gene discussed is TNF; the disease is myocardial infarction.