Among the additional variants related to neurodevelopmental disorders identified by Trio-WES, ALDH18A1 (NM_002860.4): c.683C>T (p.P228L) and ASH1L (NM_018489.3): c.6238G>A (p.V2080I) were de novo and paternally inherited, respectively, but did not fulfill the criteria for co-segregation. Here, ALDH18A1 is linked to neurodevelopmental disorder.