As shown in Figure 3a, the frequency of IL-2+ cells within CD3+, CD4+, and CD8+ T cell populations significantly decreased after coincubation with human melanoma A375 cells regardless of IFN-γ pre-exposure (p < 0.05), while no significant change was seen after coincubation with Hermes 1 cells (Supplementary Figure S3). Here, IL2 is linked to melanoma.