All samples tested negative for multiplex sVNT (Figure 4), confirming the absence of serologic evidence for ACE-2-using coronaviridae (RaTG13, RsSHC014, WIV-1, CoV Rs2018B) in the bat guano collectors prior to the COVID-19 pandemic and providing an important confirmatory test of pre-SARS-CoV-2 seronegativity. This evidence concerns the gene ACE2 and COVID-19.