Real-time PCR analysis of mRNA from whole brain tissue revealed elevated expression of proinflammatory mediators ll-6, Il-1β, Ccl2, Cxcl9, and Cxcl10 (Figure 3A–E) in both the WT and eNOS+/− mice 3 days post-MA10 infection. The gene discussed is CXCL9; the disease is infection.