RGS14 and contagious pustular dermatitis: In the challenge protection experiment, both rGS14-QuadMut and rGS14-TrypMut-immunized groups did not show typical clinical symptoms of orf at the scratch inoculation site and lips, while the control group developed pustules and other typical pathological features of orf at the scratch inoculation site and lips, indicating that both rGS14-QuadMut and rGS14-TrypMut effectively induced immune protection.