Tumour cell invasion into the collagen-rich interstitial tissue was longitudinally monitored 4–11 days post-implantation; 3D tissue constituents, including fibrillar collagen and myofibers (second-harmonic generation (SHG)), macrophages and blood vessels (70-kD dextran-Alexa Fluor 750), were corecorded alongside monitoring the localization of GFP-anillin in the nucleus and cytoplasm. The gene discussed is ANLN; the disease is neoplasm.