mCherry-labelled HT-1080 cells coexpressing WT GFP-anillin and HA-Ect2 (ANLN/Ect2 (WT)) or anillin and Ect2 dual mutant (ANLN-Δ3/Ect2-DHmut; Extended Data Fig. 4k) were injected between the chick embryo chorioallantoic membrane (CAM) ectoderm and endoderm layers, and their invasion was monitored 5 days post-tumour cell injection using intravital imaging. This evidence concerns the gene ANLN and neoplasm.