High-magnification imaging of cells expressing dual WT GFP-anillin and HA-Ect2 (Fig. 6a–c) reveals that, in agreement with the data obtained from mouse in vitro maturation (Fig. 4), a higher proportion of cells that invaded out of the primary tumour displayed anillin accumulation at the cell edges compared with those remaining inside the tumour core (Fig. 6d). This evidence concerns the gene ANLN and neoplasm.