To confirm this universal role of ITGB1 in nHEV entry, we transfected other hepatoma and non-hepatoma cell lines, such as Huh-7, HepG2/C3A, lung A549, and intestinal Caco-2 cells with small interfering RNA (siRNA) targeting ITGB1 (siITGB1) or non-targeting siRNA (siNT) 48 h prior to HEV inoculation. Here, ITGB1 is linked to hepatocellular carcinoma.