To quantify these cells following infection with each F. oxysporum strain, we dissected corneas that were infected with MRL8996 or Fol4287, digested them with collagenase, and incubated the infiltrating cells with antibodies against CD45 and CD11b (which recognize total myeloid cells) and Ly6G and Ly6C (which identify neutrophils and monocytes, respectively) (39). The gene discussed is ITGAM; the disease is infection.