As a first step to understanding RAS-dependent regulation of RALA S194 phosphorylation and its role in cancers, RAS-independent (MCF7 and SKOV3) and RAS-dependent (T24, UMUC3 and MiaPaCa2) cell lines were chosen for study based on their known RALA expression and activation status (Lim et al., 2010; Pawar et al., 2016; Oxford et al., 2005). Here, RALA is linked to cancer.