In evaluating the RALA S194 phosphorylation using anti-phospho-RALA antibody (Serine 194 residue) (Merck-Millipore, 07-2119) in multiple cancer cell lines, we discovered that pS194RALA detection in certain RAS-dependent cancer cell lines (T24, UMUC3 and MiaPaCa2) could be non-specific. Here, RALA is linked to cancer.