The known genes in CRC were frequently mutated both in primary CRC of NMF_cos1 or NMF_cos2, including KRAS, TP53, APC, PCDHB12, ZNF160, LRP2, FAT4, MUC16, and ARID1A, however, the mutation rates of these genes were different. The gene discussed is KRAS; the disease is colorectal carcinoma.