first observed ASC interactions with AIM2, ZBP1, pyrin, CASP1, CASP8, RIPK3, RIPK1, and FADD through immunoprecipitation after Herpes simplex virus (HSV1) and Francisella novicida infections; While 12 hours post-infection, ASC spots co-localized with AIM2, ZBP1and pyrin within the same cells, suggesting AIM2-PANoptosome formation during infection. Here, ZBP1 is linked to infection.