Automated annotation via SingleR revealed 22 cell subpopulations, including T cells (CD4 Memory T, CD8 T, CD4 + Treg), myeloid cells (Neutrophils, Alveolar M, cDC2/moDCs, Lipid-associated M, pDCs), B cells (B cell, Plasma, B activated), malignant epithelial cells (SOX2 Cancer, CXCL1 Cancer, CDKN2A Cancer, Proliferating Cancer), normal epithelial cells (Alveolar, Ciliated), fibroblasts (SMC, CAF), other immune cell types (NK, Mast), and stressed/unknown cells (Figures 3D,E). This evidence concerns the gene CXCL1 and cancer.