Western blotting showed that NF-α1/CPE and NF-α1/CPE-E342Q expression was increased in 3xTg -AD mice treated with AAV-NF- α1/CPE and AAV-NF-α1/CPE-E342Q in comparison with those treated with GFP (Fig 2A). This evidence concerns the gene POU2F1 and Alzheimer disease.