RNA-seq analyses showed that DEN-induced Srsf3 KO liver cancer in both female and male mice exhibited a significantly reduced expression of Foxa1/2/3 and ERα than DEN-induced Srsf3 WT liver cancer (Figure 5A), which could be verified by quantitative RT-PCR (Figure 5B, Figure S12A). This evidence concerns the gene FOXA1 and liver cancer.