To understand how Srsf3 KO in mammary glands and liver tissues could have an opposite effect on carcinogenesis which might associated with altered gene expression and RNA splicing in two different tissues, we conducted RNA-Seq for the Erbb2-induced breast cancer tissues with Srsf3 KO and Srsf3 WT (Figure S5A–S5B, Table S1), and DEN-induced liver cancer tissues with Srsf3 KO and WT Srsf3 (Figure S5D–S5E, Table S2). Here, ERBB2 is linked to breast cancer.